Name of cell: Bone Marrow-Derived Mesenchymal Stem Cells

Abbreviation: BMSCs

Species: Human

Source: Bone Marrow

Morphology: Fibroblast-like, CFU positive

Culture Medium: Complete DMEM/low glucose (cDMEM/LG): DMEM/LG with 15 %(v/v) Fetal Bovine Serum (FBS), 1X non-essential amino acids, 2 Mm L-glutamine,100 U/mL penicillin, 100 μg/mL streptomycin, and 0.25 μg/mL fungizone.

Preservation Medium: 90% FBS + 10% DMSO

Sterility: Tests for mycoplasma, bacteria and fungi were negative.

Viability: 98%

Reference:

1. Somaieh Kazemnejad, Mohammad-Mehdi Akhondi, Masoud Soleimani, Amir Hassan Zarnani, Manijeh Khanmohammadi, Saeedeh Darzi. Characterization and chondrogenic differentiation of menstrual blood- derived stem cells on a nanofibrous scaffold. The Int J of Artificial Organs, 2012, 35(1):55-66.

2. Sayeh Khanjani, Manijeh Khanmohammadi, Amir-Hassan Zarnani, Mohammad-Mehdi Akhondi, Ali Ahani, Zahra Ghaempanah, Mohammad Mehdi Naderi, Saman Eghtesad, Somaieh Kazemnejad. Comparative evaluation of differentiation potential of menstrual blood- versus bone marrow- derived stem cells into hepatocyte-like cells. PLoS ONE, 2014, 9(2): e86075.

3. Xu, W., Zhang, X., Qian, H., et al. (2004). Mesenchymal stem cells from adult human bone marrow differentiate into a cardiomyocyte phenotype in vitro. Experimental Biology and Medicine, 229, 623–631.

Name of cell: Endometrial Stem Cells

Abbreviation: EnSCs

Species: Human

Source: Endometrium tissue

Morphology: Fibroblast-like, CFU positive

Culture Medium: Complete DMEM/F-12 (cDMEM/F-12): DMEM/F-12 with 15 %(v/v) Fetal Bovine Serum (FBS), 1X non-essential amino acids, 2 Mm L-glutamine,100 U/mL penicillin, 100 μg/mL streptomycin, and 0.25 μg/mL fungizone.Preservation Medium: 90% FBS + 10% DMSO

Sterility: Tests for mycoplasma, bacteria and fungi were negative.

Viability: 98%

Reference:

1. Grewal S, Carver J, Ridley AJ, Mardon HJ. 2010. Human endometrial stromal cell rho GTPases have opposing roles in regulating focal adhesion turnover and embryo invasion in vitro. Biol Reprod, 83, 75-82.

2. Carver J, Martin K, Spyropoulou I, Barlow D, Sargent I, Mardon H. 2003. An in-vitro model for stromal invasion during implantation of the human blastocyst. Hum Reprod, 18, 283-290.

3. Delbandi AA, Mahmoudi, Shervin, Zarnani. 1,25-Dihydroxy Vitamin D3 Modulates Endometriosis-Related Features of Human Endometriotic Stromal Cells. Am J Reprod Immunol, 2015.

Name of cell: Amniotic Membrane-Derived Epithelial Stem Cells

Abbreviation: AECs

Species: Human

Tissue: Amniotic Membrane

Morphology: Fibroblast-like

Culture Medium: Complete RPMI (cRPMI): RPMI with 10 %(v/v) Fetal Bovine Serum (FBS), 1X non-essential amino acids, 2 Mm L-glutamine,100 U/mL penicillin, 100 μg/mL streptomycin, and 0.25 μg/mL fungizone.

Preservation Medium: 90% FBS + 10% DMSO

Sterility: Tests for mycoplasma, bacteria and fungi were negative.

Viability: 98%

Reference:

1. Kang NH, Yi BR, Lim SY, Hwang KA, Baek YS, Kang KS, Choi KC. Human amniotic membrane-derived epithelial stem cells display anticancer activity in BALB/c female nude mice bearing disseminated breast cancer xenografts. Int J Oncol. 2012 Jun;40(6):2022-8.

2. M Tabatabaei, N Mosaffa, Shohreh Nikoo, Mahmood Bozorgmehr, Roya Ghods, Somaieh Kazemnejad, S Rezania, B Keshavarzi, S Arefi, F Ramezani-Tehrani, Ebrahim Mirzadegan, Amir Hassan Zarnani. Isolation and partial characterization of human amniotic epithelial cells: the effect of trypsin. Avicenna J Med Biotechnol. 2014 6(1):10-20.